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Position Description:
Primary Functions
• Reprogram human somatic cell lines into induced pluripotent stem cells (iPSCs).
• Monitor inventory and maintain supply of inventory.
• Maintain iPSCs in culture.
• Differentiate iPSCs into cardiomyocytes (CMs), neurons, beta islet cells and other cell types.
• Provide quality control of generated iPSCs and differentiated iPSCs.
• Bank and prepare iPSCs and differentiated iPSCs.
• Educate other technicians in cell culture techniques.
• Desirable skills include flow cytometry, qRT-PCR, confocal microscopy, primary cell isolation, antibody validation, transfection, and protein over-expression and CRISPR technologies.
• Handling various human somatic cells (e.g., fibroblasts, peripheral blood mononuclear cells, urine) and tissues (e.g., skin biopsy and heart tissue).
• Strong organizational skills, passion for methodological development, and understanding of basic experimental design are required for this position.
• Participate in meetings with Cardiovascular Center leadership, principal investigator and research staff and evaluate/interpret the validity of data as needed.
• Oversee daily lab operation in the absence of the supervisor or Principal Investigator.
• Assist in development and implementation of research protocols.
• Record and compile accurate records and communicate summaries of experimental results.
• Help evaluate, interpret, and assess experimental data and quality control of assays.
• Operate, calibrate and perform routine maintenance on specialized equipment used in research.
• Other duties and projects as assigned.
Preferred Schedule:
Full-time role with expectations for coverage during core business hours and flexibility required as necessary to accommodate business needs
Position Requirements:
Specifications
Appropriate experience may be substituted for education on equivalent basis
Minimum Required Education: Bachelor’s degree
Minimum Required Experience: 1 year of cell culture work experience in a stem cell research laboratory with human induced pluripotent stem cells (iPSCs).
Preferred Education:
Preferred Experience:
Field:
MCW as an Equal Opportunity Employer and Commitment to Non-Discrimination
The Medical College of Wisconsin (MCW) is an Equal Opportunity Employer. We are committed to fostering a diverse community of outstanding faculty, staff, and students, as well as ensuring equal educational opportunity, employment, and access to services, programs, and activities, without regard to an individual’s race, color, national origin, religion, age, disability, sex, gender identity/expression, sexual orientation, marital status, pregnancy, predisposing genetic characteristic, or military status. Employees, students, applicants or other members of the MCW community (including but not limited to vendors, visitors, and guests) may not be subjected to harassment that is prohibited by law or treated adversely or retaliated against based upon a protected characteristic.
